Toxoplasmosis is an infection caused by a parasite called Toxoplasma gondii. It is estimated that 1/3 of the global population has the parasite living in their brains. Most people do not recognize any symptoms. If infection occurs for the first time during pregnancy, the parasite can pass from the mother to the baby. Passage of the parasite causes developmental delays, seizures, and retinal damage that causes loss of sight. A monthly screening for antibodies against the parasite in pregnant women (to identify mothers at risk for passing the parasite to their babies), as is the standard for some European countries, combined with treatment, can prevent suffering and save lives. Chronic toxoplasma infection can pose risks to a mother and her fetus, but this has not been well studied. Data from the Chicago Multiethnic Prevention and Surveillance Study (COMPASS) found that women who are seropositive for toxoplasma had a higher chance of gestational diabetes. The purpose of this study is to further explore this relationship in the South Side of Chicago and in Tinghir, a desert region of the southeastern of Morocco with a high seroprevalence (~50%). This proposal builds on groundwork laid by an earlier work in Morocco, supported by IIRP Faculty grant, where we implemented one of the largest studies ever performed to assess the feasibility of monthly Point-of-Care (POC)-test based prenatal screening for Toxoplasma infections in the nomad population, a vulnerable, understudied population that suffers many adverse Social Determinants of Health. It is one of the only studies that will follow patients across real time to study their outcomes. We will investigate the cellular and immunological mechanisms that may influence the association between gestational diabetes and toxoplasma seropositivity, and how this impacts the severity of the disease in patients.